Journal: Scientific Reports

Article Title: Genetic editing of primary human dorsal root ganglion neurons using CRISPR-Cas9

doi: 10.1038/s41598-025-91153-2

Figure Lengend Snippet: Lipofectamine 3000 reagent protocol. The hDRG cells were transfected according to these optimized specifications.

Article Snippet: BrainPhys ® media (Stemcell technologies, cat. no. 05790), 1% penicillin/streptomycin (Thermo Fisher Scientific, Cat# 15070063), 1% GlutaMAX ® (United States Biological, cat. no. 235242), 2% NeuroCultTM SM1 (Stemcell technologies, cat. no. 05711), 1% N-2 Supplement (Thermo Scientific, cat. no. 17502048), 2% HyCloneTM Fetal Bovine Serum (ThermoFisher Scientific SH3008803IR), 1: 1000 FrdU, 10 ng/mL Human β nerve growth factor - (Cell Signaling Technology, Cat# 5221SC).

Techniques: Transfection

Journal: Nature Communications

Article Title: BrainPhys neuronal medium optimized for imaging and optogenetics in vitro

doi: 10.1038/s41467-020-19275-x

Figure Lengend Snippet: a The osmolality of BrainPhys Imaging (BPI) is similar to human cerebrospinal fluid (hCSF) (~305 mOsmol/kg). Neurobasal and NEUMO were significantly lower (210–220 mOsmol/kg) and DMEM/F12 and FluoroBrite higher (317–338 mOsmol/kg). Media osmolality data was collected from four replicates per condition. A total of three hCSF samples, each pooled from up to four subjects, were tested across three independent experiments ( n = 3). See also Supplementary Fig. . b – f Comparison of the optical properties of BPI with other basal media specialized for imaging (NEUMO, FluoroBrite), standard basal neuromedia (BrainPhys, BP; BrainPhys without phenol red, BP no PR; DMEM/F12; Neurobasal) and control media (phosphate-buffered saline, PBS; deionized water, H 2 O). b The absorbance spectra from 300 to 800 nm acquired from basal media alone (without cells), and after adding supplements required for the long-term culture of brain cells. Virtually all fluorophores used for cell imaging require light stimulation above 300 nm. c – f The mean autofluorescence intensities of basal media (without cells) across the entire visible spectrum. BPI shows autofluorescence intensities similar to PBS. c The emission spectra across 400–700 nm captured for the 375 (ultra-violet), 405 (violet), 488 (blue), and 532 nm (green) excitation wavelengths from test and control media. d – f Autofluorescence at 460, 520, and 590 nm emission wavelengths were measured following excitation at 355, 485, and 544 nm, respectively. Results were generated from eight replicate wells per medium ( n = 8) analyzed across three independent experiments. For normalization, the mean fluorescence intensity in PBS was subtracted from the other media. Data are presented as mean ± SEM. Significance determined via two-tailed nonparametric unpaired (Mann–Whitney) tests. ns, P > 0.05.

Article Snippet: BrainPhys™ (Cat. No. 05790, STEMCELL Technologies), BrainPhys™ Imaging (Cat. No. 05796, STEMCELL Technologies), BrainPhys without Phenol Red (Cat. No. 05791, STEMCELL Technologies), FluoroBrite™ DMEM (Cat. No. A1896701, Thermo Fisher Scientific), DMEM/F12 (Cat. No. 10565018, Thermo Fisher Scientific), Neurobasal (Cat. No. 21103-049, Thermo Fisher Scientific), and BrightCell™ NEUMO (Cat. No. SCM146, Merck) were obtained via the source and catalog details provided.

Techniques: Imaging, Generated, Fluorescence, Two Tailed Test, MANN-WHITNEY